In Indian scenario, there are number of plant resources which possessed potential medicinal properties. Immune stimulants from plant resources contained variety of therapeutic agents which are really novel and influenced in controlling various diseases of living organisms including fish and shrimp. Herbal extracts are involved in regulating different defence properties mainly, antiseptic, antibacterial, antiviral, antimycotic, anti-toxigenic, antiparasitic, anticancer, antioxidant and insecticidal. LiO was developed in an herbal liquid based nano concept that controls many pathogenic infections including bacteria, fungus and parasites in the aquaculture ponds.